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UDC 638.15.-085.152:619

RESEARCH DISINFECTING EFFECT OF UV IN THE CONSERVATION ACTION POTENTIAL OF BEE COLONIES

N. A. Romanchenko, I. G. Masli, N. P. Kundenko, Y. K. Sanin, Е. С. Tsekhmeister

Kharkiv National Technical University of Agriculture named Petro Vasilenko

Key words: UV radiation, Paenibacillus larvae, subsp. larvae, American foulbrood

The article presents the results of studies of the effect of UV irradiation on the controversy Paenibacillus larvae larvae. It was found that the spores of bacteria Paenibacillus larvae, subsp. larvae have sensitive to UV radiation having a wavelength in the range 254 nm.

Disinfection is an important part of prevention and control of communicable diseases in beekeeping. The main factors of transmission of pathogens is Wax brood, feed stocks, contaminated spores, tools, spare cells, beehives, honey. Disputes pathogen particularly dangerous disease brood bees – American foul brood – sticks Paenibacillus larvae, subsp. larvae are particularly resistant to physical and chemical factors, if they are in films remains, honey, bee brad and wax. According to the literature to data spores are killed in water temperature of 90 0 C in 3 hours and 95 0C – an hour. Boiling corpses larvae in water for 1 minute 80% spores are killed, 5 min. – 99 % and 14 min. – All.

Objective: To determine the disinfecting properties of UV exposure to the test objects contaminated with spores of the bacteria Paenibacillus larvae, subsp. larvae in laboratory and production environments.

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Materials and methods. Disinfectant effect of UV irradiation was studied in test sites – splicing, Wax, artificially contaminated with spores of the bacteria Paenibacillus larvae, subsp. Larvae, as well as combs, selected from families affected hnyltsevymy diseases of bees.

During clinical studies was used isolate strain Paenibacillus larvae, subsp. larvae, isolated from the patient American foulbrood of bees. For spores Paenibacillus larvae, subsp. larvae prepared with washed cells containing dead larvae from the disease.

Based on the analysis of scientific literature on the issues of development technologies and ways to combat pathogenic microflora bees preferred physical means to combat the use of short-wave UV radiation range of optical radiation.

The effectiveness of bactericidal effect UV radiation wavelength, intensity of exposure, duration of exposure, species belonging cultivated microorganisms, the distance from the source. It is difficult to consider all options that could influence once the entire spectrum of microorganisms.

The effectiveness of UV radiation to disinfect surfaces in each case is calculated taking into account all the parameters that affect the process of irradiation microorganisms.

UV radiation is highly active when microorganisms are located on the surface of objects processed in one layer. For the location of the upper multilayer protect those under them (shielding phenomenon).

The most effective way to deal with bees pathogenic microflora is a dynamic way of irradiation of biological objects, providing a complete destruction of at Exposure 1.2 104 mW s/cm2 and speed of the irradiation facility of 0.01 m s.

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