Распознавание генов (стр. 3 )

GENSCAN 1.0 Date run: 25-Nov-107 Time: 11:09:36

Sequence Cjac(homolog_of=Hum:8365) : 8650 bp : 62.27% C+G : Isochore 4 C+G%)

Parameter matrix: HumanIso. smat

Predicted genes/exons:

Gn. Ex Type S. Begin...End. Len Fr Ph I/Ac Do/T CodRg P.... Tscr..

--- ----

1.01 Intr + 3 26.89

1.02 Intr + 1 25.76

1.03 Intr + 3 15.56

1.04 Intr + 4 5.14

1.05 Intr + 4 16.77

1.06 Intr + 5 12.21

1.07 Intr + 5 16.44

1.08 Intr + 591.20

1.09 Intr + 6 .51

1.10 Intr + 6 9 19.92

1.11 Term + 6 8.33

1.12 PlyA + 6

2.06 PlyA - 6

2.05 Term - 77691

2.04 Intr - 7 16.94

2.03 Intr - 7 9 32.26

2.02 Intr - 8 38.12

2.01 Init - 857.97

Предсказываемая(ые) последовательность(и):

>Cjac(homolog_of=Hum:8365)|GENSCAN_predicted_peptide_1|741_aa

LGSAAGKASAMTLIEGVGDEVTVLFSVLACLLVLALAWVSTHTAEGGDPLPQLSGTPSPA

QPSAAMAATDSMRGEAPGPETPSLRHRGQAAQPEPSTGVTATPTQPALDSPQEPLVLRLK

FLNDSEQVARAWPHDTIGSLKRTQFPGREQQVRLIYQGQLLGDDTQTLGSLHLPPNCVLH

CHVSTRVGPPSPPCPPGSEPGPSGLEIGSLLLPLLLLLLLLLWYCQIQYRPFFPLTATLG

LAGFTLLLSLLAFAMYRPKWGDWPPGGGPSTGHVQGLQRLLEQARSPGELLRWLGQNPTK

VRAHHYPVALRRLGQLLGSRPRPPPVEQATLQDLSQLIIRNCPAFDMHTIHVCLHLAVLL

GFPADGPLVRALEQERRLRLPPKPPPPLSSLLRGGQRLEAALSCPRFLRYPRQHLISSLA

ETRPEELTPHVMVLLAQHLARHRLREPQLLEAIARFLVVQENQLSSKVVQKLVLPFGRLN

YLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFRALHFVFSPGFINYISGT

PHALIVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKYSHKDIVAEG

LRQLLGEEKYRQDLTVPPGYCTDFLLCVGSSGAVLPVRTQDPFLPYPPRSCPQGQAACSP

TTRDPAQRVVLVLRERWHFCRDGRVLLGSRALRERHLGLMGYQLLPLPFEELESQRGLPQ

LKSYLRQKLQALGLRWGPEGG

>Cjac(homolog_of=Hum:8365)|GENSCAN_predicted_peptide_2|248_aa

MVASLLPAILVFILIFMETQITTLIISKKERMLQKGSGFHLDLLLIVAMGGICALFGLPW

LAAATVRSVTHANALTVMSKAVAPGDKPKIQEVKEQRVTGLLVALLVGLSIVIGDVLRQI

PLAVLFGIFLYMGVTSLNGIQFYERLHLLLMPPKHHPDVTYVKKVRTLRMHLFTALQLLC

LALLWAVMSTAASLAFPFILILTVPLRMVVLTRVFTEREMKCLDANEAEPVFDEREGVDE

YNEMPMPV

Иллюстрация, выдаваемая самой программой:

Задание №2:

С помощью программы BLASTX определить экзонно-интронную структуру мармозетки.

Результат №2:

Полное выравнивание первого:

>ref|NP_.1| Fas-activated serine/threonine kinase [Canis lupus familiaris] Length=549 GENE ID: 482802 FASTK | Fas-activated serine/threonine kinase [Canis lupus familiaris] (10 or fewer PubMed links) Score = 266 bits (680), Expect = 4e-69 Identities = 127/%), Positives = 133/%), Gaps = 0/141 (0%) Frame = +2 Query 3452 SPSPNSMLRVLLSAQTSPTRLSGLLVIPPVQPCCLGPSKWGDWPPGGGPSTGHVQGLQRL 3631 SPSPNSMLR+LLSAQ S RLSGLL++PPVQPCCLGPSKWGD PPGGG G VQGLQRL Sbjct 29 SPSPNSMLRLLLSAQASAARLSGLLLLPPVQPCCLGPSKWGDQPPGGGLHAGPVQGLQRL 88 Query 3632 LEQARSPGELLRWLGQNPTKVRAHHYPVALRRLGQLLGSRPRPPPVEQATLQDLSQLIIR 3811 LEQARSPGELLRWLGQNPTKVRAHHYPVALRRLGQLLGS+PRPPPVEQATLQDLSQLIIR Sbjct 89 LEQARSPGELLRWLGQNPTKVRAHHYPVALRRLGQLLGSQPRPPPVEQATLQDLSQLIIR 148 Query 3812 NCPAFDMHTIHVCLHLAVLLG 3874 NCP+FD+HTIHVCLHLAVLLG Sbjct 149 NCPSFDIHTIHVCLHLAVLLG 169 Score = 151 bits (381), Expect(2) = 2e-61 Identities = 74/77 (96%), Positives = 76/77 (98%), Gaps = 0/77 (0%) Frame = +3 Query 5268 LPSQVVQKLVLPFGRLNYLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFR 5447 L S+VVQKLVLPFGRLNYLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFR Sbjct 272 LSSKVVQKLVLPFGRLNYLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFR 331 Query 5448 ALHFVFSPGFINYISGT 5498 ALHFVFSPGFIN+ISGT Sbjct 332 ALHFVFSPGFINHISGT 348 Score = 111 bits (277), Expect(2) = 2e-61 Identities = 52/54 (96%), Positives = 53/54 (98%), Gaps = 0/54 (0%) Frame = +2 Query 5588 TGTPHALIVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKY 5749 +GTPHA IVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKY Sbjct 346 SGTPHAFIVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKY 399 Score = 119 bits (298), Expect(2) = 4e-41 Identities = 78/%), Positives = 87/%), Gaps = 61/%) Frame = +1 Query 5974 SHKDIVAEGLRQLLGEEKYRQDLTVPPGY------CTGERGASGRPRETEPWLRPPALLP 6135 SHKDIVAEGLRQLLGEEKYRQDLTVPPGY C GA +LP Sbjct 400 SHKDIVAEGLRQLLGEEKYRQDLTVPPGYCTDFLLCVSSSGA----VLP 444 Query 6136 LQTSCCVSAALVLCFP*EPRTPSCHTHQGPAHRDRLPVAPLPEILPRGKGGRVGEPWPPC 6315 ++T +P P P P+G+ P Sbjct 445 VRTQ--DPFLPYP------PRSCPQGQAASQSTTHDPA 474 Query 6316 PPCPEPCPSPHRVVLVLRERWHFCRDGRVLLGSRALRERHLGLMGYQLLPV 6468 RVVL+LRERWHFCRDG+VLLGSRALRERHLGLMGYQLLP+ Sbjct QRVVLMLRERWHFCRDGQVLLGSRALRERHLGLMGYQLLPL 515 Score = 75.5 bits (184), Expect(2) = 4e-41 Identities = 35/35 (100%), Positives = 35/35 (100%), Gaps = 0/35 (0%) Frame = +3 Query 6546 LPFEELESQRGLPQLKSYLRQKLQALGLRWGPEGG 6650 LPFEELESQRGLPQLKSYLRQKLQALGLRWGPEGG Sbjct 515 LPFEELESQRGLPQLKSYLRQKLQALGLRWGPEGG 549 Score = 162 bits (410), Expect = 8e-38 Identities = 93/%), Positives = 96/%), Gaps = 47/%) Frame = +1 Query 4348 GFPADGPLVRALEQERRLRLPPKPPPPLSSLLRGGQRLEAALSCPRFLRYPRQHLISSLA 4527 GFP+DGPL+ ALEQERR RLPPKPPP L +L GGQRLEAALSCP FLR PRQHLI SLA Sbjct 169 GFPSDGPLMCALEQERRFRLPPKPPPTLQPVLHGGQRLEAALSCPHFLRRPRQHLIRSLA 228 Query 4528 GAGGA*RQIGMRRADRRRKVGSVS*AEVELSQLEVRPEAHCDRLFFTETRPEELTPHVMV 4707 E RPEELTPHVMV Sbjct --EARPEELTPHVMV 241 Query 4708 LLAQHLARHRLREPQLLEAIARFLVVQENQLSSKV 4812 LLAQHLARHRLREPQLLEAIA FLVVQE QLSSKV Sbjct 242 LLAQHLARHRLREPQLLEAIAHFLVVQEAQLSSKV 276 Score = 58.5 bits (140), Expect = 2e-06 Identities = 25/26 (96%), Positives = 25/26 (96%), Gaps = 0/26 (0%) Frame = +3 Query 2001 MRRPRGEPGPRAPRPTEGATCAGPGE 2078 MRRPRGEPG RAPRPTEGATCAGPGE Sbjct 1 MRRPRGEPGSRAPRPTEGATCAGPGE 26

Полное выравнивание второго:

>ref|XP_860611.1| PREDICTED: similar to Fas-activated serine/threonine kinase (FAST kinase) isoform 4 [Canis familiaris] Length=522 GENE ID: 482802 FASTK | Fas-activated serine/threonine kinase [Canis lupus familiaris] (10 or fewer PubMed links) Score = 266 bits (680), Expect = 4e-69 Identities = 127/%), Positives = 133/%), Gaps = 0/141 (0%) Frame = +2 Query 3452 SPSPNSMLRVLLSAQTSPTRLSGLLVIPPVQPCCLGPSKWGDWPPGGGPSTGHVQGLQRL 3631 SPSPNSMLR+LLSAQ S RLSGLL++PPVQPCCLGPSKWGD PPGGG G VQGLQRL Sbjct 29 SPSPNSMLRLLLSAQASAARLSGLLLLPPVQPCCLGPSKWGDQPPGGGLHAGPVQGLQRL 88 Query 3632 LEQARSPGELLRWLGQNPTKVRAHHYPVALRRLGQLLGSRPRPPPVEQATLQDLSQLIIR 3811 LEQARSPGELLRWLGQNPTKVRAHHYPVALRRLGQLLGS+PRPPPVEQATLQDLSQLIIR Sbjct 89 LEQARSPGELLRWLGQNPTKVRAHHYPVALRRLGQLLGSQPRPPPVEQATLQDLSQLIIR 148 Query 3812 NCPAFDMHTIHVCLHLAVLLG 3874 NCP+FD+HTIHVCLHLAVLLG Sbjct 149 NCPSFDIHTIHVCLHLAVLLG 169 Score = 151 bits (381), Expect(2) = 2e-61 Identities = 74/77 (96%), Positives = 76/77 (98%), Gaps = 0/77 (0%) Frame = +3 Query 5268 LPSQVVQKLVLPFGRLNYLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFR 5447 L S+VVQKLVLPFGRLNYLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFR Sbjct 245 LSSKVVQKLVLPFGRLNYLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFR 304 Query 5448 ALHFVFSPGFINYISGT 5498 ALHFVFSPGFIN+ISGT Sbjct 305 ALHFVFSPGFINHISGT 321 Score = 111 bits (277), Expect(2) = 2e-61 Identities = 52/54 (96%), Positives = 53/54 (98%), Gaps = 0/54 (0%) Frame = +2 Query 5588 TGTPHALIVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKY 5749 +GTPHA IVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKY Sbjct 319 SGTPHAFIVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKY 372 Score = 119 bits (298), Expect(2) = 4e-41 Identities = 78/%), Positives = 87/%), Gaps = 61/%) Frame = +1 Query 5974 SHKDIVAEGLRQLLGEEKYRQDLTVPPGY------CTGERGASGRPRETEPWLRPPALLP 6135 SHKDIVAEGLRQLLGEEKYRQDLTVPPGY C GA +LP Sbjct 373 SHKDIVAEGLRQLLGEEKYRQDLTVPPGYCTDFLLCVSSSGA----VLP 417 Query 6136 LQTSCCVSAALVLCFP*EPRTPSCHTHQGPAHRDRLPVAPLPEILPRGKGGRVGEPWPPC 6315 ++T +P P P P+G+ P Sbjct 418 VRTQ--DPFLPYP------PRSCPQGQAASQSTTHDPA 447 Query 6316 PPCPEPCPSPHRVVLVLRERWHFCRDGRVLLGSRALRERHLGLMGYQLLPV 6468 RVVL+LRERWHFCRDG+VLLGSRALRERHLGLMGYQLLP+ Sbjct QRVVLMLRERWHFCRDGQVLLGSRALRERHLGLMGYQLLPL 488 Score = 75.5 bits (184), Expect(2) = 4e-41 Identities = 35/35 (100%), Positives = 35/35 (100%), Gaps = 0/35 (0%) Frame = +3 Query 6546 LPFEELESQRGLPQLKSYLRQKLQALGLRWGPEGG 6650 LPFEELESQRGLPQLKSYLRQKLQALGLRWGPEGG Sbjct 488 LPFEELESQRGLPQLKSYLRQKLQALGLRWGPEGG 522 Score = 88.6 bits (218), Expect = 1e-15 Identities = 45/48 (93%), Positives = 45/48 (93%), Gaps = 0/48 (0%) Frame = +1 Query 4669 ETRPEELTPHVMVLLAQHLARHRLREPQLLEAIARFLVVQENQLSSKV 4812 E RPEELTPHVMVLLAQHLARHRLREPQLLEAIA FLVVQE QLSSKV Sbjct 202 EARPEELTPHVMVLLAQHLARHRLREPQLLEAIAHFLVVQEAQLSSKV 249 Score = 58.5 bits (140), Expect = 2e-06 Identities = 25/26 (96%), Positives = 25/26 (96%), Gaps = 0/26 (0%) Frame = +3 Query 2001 MRRPRGEPGPRAPRPTEGATCAGPGE 2078 MRRPRGEPG RAPRPTEGATCAGPGE Sbjct 1 MRRPRGEPGSRAPRPTEGATCAGPGE 26 Score = 55.1 bits (131), Expect = 2e-05 Identities = 29/55 (52%), Positives = 34/55 (61%), Gaps = 3/55 (5%) Frame = +1 Query 4348 GFPADGPLVRALEQERRLRLPPKPPPPLSSLLRGGQRLEAALSCPRFLRYPRQHL 4512 GFP+DGPL+ ALEQERR RLPPKPPP L +L + E P + QHL Sbjct 169 GFPSDGPLMCALEQERRFRLPPKPPPTLQPVLHEARPEELT---PHVMVLLAQHL 220

GENE ID: 482802 FASTK | Fas-activated serine/threonine kinase [Canis lupus familiaris], +
координаты по белку	координаты по ДНК

1) начало экзона 1	начало экзона 2001
конец экзона 26	конец экзона 2078

2) начало экзона 29	Начало экзона 3452
конец экзона 169	конец экзона 3874

3) начало экзона 169	начало экзона 4348
конец экзона 228	конец экзона 4527
4) начало экзона 229	начало экзона 4668
конец экзона 269	конец экзона 4812

5) начало экзона 272	начало экзона 5268
конец экзона 348	конец экзона 5498
6) начало экзона 346	начало экзона 5588
конец экзона 399	конец экзона 5749
7) начало экзона 374	начало экзона 5974
конец экзона 402	конец экзона 6135
8) начало экзона 451	начало экзона 6341
конец экзона 489	конец экзона 6465
9) начало экзона 515	начало экзона 6546
конец экзона 549	конец экзона 6650

GENE ID: 482802 FASTK | Fas-activated serine/threonine kinase [Canis lupus familiaris], +
координаты по белку	координаты по ДНК

1) начало экзона 346	начало экзона 2001
конец экзона 399	конец экзона 2048

2) начало экзона 29	начало экзона 3452
конец экзона 169	конец экзона 3874

3) начало экзона 202	начало экзона 4669
конец экзона 249	конец экзона 4812
4) начало экзона 245	начало экзона 5268
конец экзона 321	конец экзона 5498

5) начало экзона 319	начало экзона 5588
конец экзона 372	конец экзона 5749
6) начало экзона 400	начало экзона 5974
конец экзона 471	конец экзона 6001
7) начало экзона 475	начало экзона 6316
конец экзона 515	конец экзона 6468
8) начало экзона 488	начало экзона 6546
конец экзона 522	конец экзона 6650